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KMID : 0363720070400020077
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Korean Journal of Anatomy
2007 Volume.40 No. 2 p.77 ~ p.83
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SP1 Mediates IFNγ-induced TIMP-1 Transctription in C6 Astroglioma Cellsㅊ
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Shin Joo-Hyoun
Kim Il-Doo
Kim Seung-Woo
Lee Min-Hyung
Lee Ja-kyung
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Abstract
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Tissue inhibitors of metalloproteinases (TIMPs) comprise a family of secreted multifunctional proteins that consists of four members (TIMP-1 to TIMP-4). TIMPs are all major inhibitors of most matrix metalloproteinases (MMPs). They are synthesized by a variety of different cells and regulated by a number of cytokines and by growth and differentiation factors. The balance between MMPs and TIMPs plays a crucial role in the turnover of extracellular matrix in normal and pathological conditions. Here, we report that the production of TIMP-1 was upregulated in interferon (IFN)γ-treated C6 astroglioma cells and that the proximal 226 bp region of the promoter of the TIMP-1 gene is responsible for IFNγ-induced induction in C6 astroglioma cells. The induction of TIMP-1 production by IFNγ was virtually abolished by introducing mutations into the putative SP1-response element in the oter, indicating that the SP1 binding site conferred responsiveness onto a heterologous promoter. Together the results suggest that the IFNγ-induced upregulation of TIMP-1 production in C6 astroglioma cells is mediated by the SP1 binding site localized in the TIMP-1 gene promoter.
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KEYWORD
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SP1 Mediates IFNγ-induced TIMP-1 Transctription in C6 Astroglioma Cells
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